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Related Experiment Videos

Blastocystis hominis: A simplified, high-efficiency method for clonal growth on solid agar.

K S Tan1, G C Ng, E Quek

  • 1Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore.

Experimental Parasitology
|October 20, 2000
PubMed
Summary

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This study introduces a novel method for culturing Blastocystis hominis (B. hominis) on agar surfaces, enabling easier cloning and expansion. This technique simplifies parasite research by allowing colonies to grow exposed on the agar.

Area of Science:

  • Microbiology
  • Parasitology
  • Cell Biology

Background:

  • Culturing protozoan parasites in agar aids axenization, cloning, and viability studies.
  • Traditional pour plate methods embed colonies within agar, making extraction difficult.
  • A surface-growth method for parasite colonies would simplify isolation and further investigation.

Purpose of the Study:

  • To report the successful culture of Blastocystis hominis (B. hominis) cells as surface colonies on solid agar.
  • To establish a simple and effective method for cloning and expansion of B. hominis.

Main Methods:

  • Culture of B. hominis on solid agar plates.
  • Enumeration of colonies and determination of plating efficiency.
  • Flow cytometry for viability assessment.

Related Experiment Videos

  • Scanning electron microscopy for colony morphology analysis.

    • Main Results:

    • B. hominis successfully cultured as dome-shaped, mucoid colonies up to 3 mm in diameter on agar.
    • Colonies remained viable for up to 2 weeks, confirmed by flow cytometry.
    • Scanning electron microscopy revealed distinct core and peripheral regions within colonies, with older colonies showing surface coat projections.

    Conclusions:

    • This study presents the first report of clonal growth of B. hominis on agar surfaces.
    • The developed method offers a simple and effective approach for cloning and expanding B. hominis.
    • Exposed colonies facilitate convenient isolation for subsequent research and analysis.