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Updated: Sep 21, 2026
An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis
Published on: September 12, 2019
[Establishment of an apoptotic model induced by adriamycin in human hepatocellular carcinoma]
Objective:
Establishment of cell apoptotic model of human hepatocellular carcinoma.
Methods:
Human cultured hepatocellular carcinoma cell line HCC-9204 was exposed in vitro to different concentrations of adriamycin for various lengths of time. Cell apoptosis was ascertained by cell morphology under transmission electron-microscope, DNA electrophoresis and flow cytometry.
Results:
When HCC-9204 cells were treated with adriamycin at 20 mumol/L for 3 hr, cell apoptosis occurred. Typical apoptotic bodies appeared and DNA ladder could be demonstrated on DNA electrophoresis. Apoptotic peak was also shown by flow cytometry. Apoptotic cells accounted for 72.1% of the cell population. Cells in G1, S and G2 phase of cell cycle were 75.9%, 24.1% and 0%, respectively. Only 35.5% of adriamycin treated cells remained alive.
Conclusion:
The apoptosis of human cultured hepatocellular carcinoma cells, HCC-9204 cell line, was successfully induced by adriamycin. Establishment of this model will help further explore the molecular mechanism of hepatoma cell apoptosis.
