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Updated: Oct 11, 2026

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

Published on: May 5, 2012

[Detection of Epstein-Barr virus in lymphoproliferative diseases by in situ hybridization]

C Xia1, F Liu, Y Sun

  • 1Department of Pathology, Beijing Chaoyang Hospital.

Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology]
|July 1, 1997
PubMed
Abstract

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...