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Updated: Aug 16, 2026

Lung Tumor Cell Recruitment Assay
Published on: February 26, 2019
[Mechanism of CD44V6 in human colorectal carcinoma metastasis]
Objective:
To study the mechanism of CD44V6 in human colorectal carcinoma (HCC) matastasis.
Methods:
The effects of CD44V6 on HCC cell adhering to base membrane and vascular endothelium were investigated by human amniotic membrane invasion model and co-culturing HCC cell with vascular endothelium. The influence of CD44V6 variants on cytoskeleton and gap junctional intercellular communication (GJIC) was studied by immunofluorescent confocal 3-dimensional reconstruction and fluorescence recovery after photobleaching technique.
Results:
CD44V6 antibody can decrease the ability of HCC cells to adhere to amniotic membrane and vascular endothelium. It also can affect the distribution, polymerization and depolymerization of actin of HCC cells, but does not significantly affect GJIC of HCC cells.
Conclusions:
CD44V6 may play an important role in promoting HCC cells to adhere to vascular endothelium and base membranes and may affect the distribution, polymerization and depolymerization of actin in HCC cells and facilitate HCC cell metastasis.
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