[Determination of mangiferin in qingqiliangying injection by reversed-phase HPLC]

A reversed-phase HPLC method has been established for the determination of mangiferin in Qingqiliangying Injection. The mangiferin was separated on a Nova-pak C18 column (150 mm x 3.9 mm) and detected at 258 nm, using methanol-tetrahydrofuran-0.85% H3PO4 aqueous solution (500:80:15) as the mobile phase. The average recovery of mangiferin was 100.9%. The method is simple, rapid, and well reproducible, and thus very reliable for the quality control of Qingqiliangying Injection.