Related Experiment Video
Updated: Aug 8, 2026

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
Published on: December 3, 2012
[Immunogold labeling electron microscopy showing vimentin filament anchored on nuclear pore complex]
Abstract:
The relationship between intermediate filament and nucleus is an important question to be solved. By combining sequential cell fractionation with immunoblotting, we showed that the intermediate filament protein in turkey erythrocyte is vimentin. Using pre-embedment immunogold labeling techniques together with sequential cell fractionation, we showed that cytoplasmic intermediate filaments in turkey erythrocyte are labeled specifically by rabbit anti-vimentin antibody-protein A-gold. Moreover, we showed that the cytoplasmic filaments anchored on nuclear pore complex were also labeled by rabbit anti-vimentin antibody-protein A-gold specifically. Our results demonstrated that cytoplasmic filaments anchored on nuclear pore complex was vimentin filament. The experiments indicated that vimentin filament may be anchored on nuclear pore complex by binding with Nup 180 and intermediate filament may be involved in nuclear transportation.
Related Concept Videos
Intralumenal Vesicles and Multivesicular Bodies
Nuclear Localization Signals and Import
Pinching-off of Coated Vesicles
Golgi Matrix Proteins
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
GPI Anchoring of Proteins in the ER Membrane
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
Immunogold Electron Microscopy

