Cellular and humoral aspects of host resistance in murine salmonellosis

Mice were challenged with a highly virulent strain of Salmonella typhimurium by intraperitoneal injections. At relatively low infecting doses, immunizations with either viable attenuated or heat killed Salm. typhimurium were found to be equally protective against otherwise fatal infections. Pre-opsonization of virulent salmonellae significantly increased the survival rate of mice infected with small numbers of the pathogen. By a cell culture method, peritoneal macrophages of mice were shown to be innately capable of destroying the ingested virulent Salm. typhimurium. Macrophages from previously infected mice did not appear to have any significant increase in their bactericidal activity against salmonellae, but they possessed cytophilic antibodies specific against the H and the O antigens of Salm. typhimurium. It is believed that humoral elements play an important role in acquired immunity in murine salmonellosis by opsonization of the pathogen.