Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cartilage and bone regeneration using gene-enhanced tissue engineering.

J M Mason1, A S Breitbart, M Barcia

  • 1Department of Research, North Shore University Hospital-New York University School of Medicine, Manhasset 11030, USA.

Clinical Orthopaedics and Related Research
|October 20, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Thermodynamic Model for Interpreting Tryptophan Excitation-Energy-Dependent Fluorescence Spectra Provides Insight Into Protein Conformational Sampling and Stability.

Frontiers in molecular biosciences·2021
Same author

Early Medical Education in the Far South.

Annals of medical history·2021
Same author

The need for longer time horizons for cost-utility evaluation in bullous pemphigoid: reply from the authors.

The British journal of dermatology·2017
Same author

Biomechanical analysis of an interference screw and a novel twist lock screw design for bone graft fixation.

Clinical biomechanics (Bristol, Avon)·2017
Same author

Doxycycline compared with prednisolone therapy for patients with bullous pemphigoid: cost-effectiveness analysis of the BLISTER trial.

The British journal of dermatology·2017
Same author

Inhibition of the spindle assembly checkpoint kinase Mps-1 as a novel therapeutic strategy in malignant mesothelioma.

Oncogene·2017

This study combined gene therapy and tissue engineering to regenerate joint cartilage, achieving significant bone and articular cartilage repair in rabbits. This novel approach offers a promising solution for cartilage defects.

Area of Science:

  • Orthopaedics
  • Regenerative Medicine
  • Biotechnology

Background:

  • Joint cartilage injuries are a significant orthopedic problem with limited effective regeneration methods.
  • Current cartilage repair procedures are costly and lack consistent reliability.
  • Developing reliable methods for joint cartilage regeneration is crucial.

Purpose of the Study:

  • To investigate the efficacy of combining gene therapy and tissue engineering for articular cartilage regeneration.
  • To assess the potential of bone morphogenic protein-7 (BMP-7) gene-modified mesenchymal stem cells in repairing osteochondral defects.
  • To establish a novel approach for regenerating damaged joint cartilage.

Main Methods:

  • Human bone morphogenic protein-7 complementary deoxyribonucleic acid was introduced into rabbit mesenchymal stem cells using a retroviral vector.

Related Experiment Videos

  • Gene-modified cells were cultured, seeded onto polyglycolic acid scaffolds, and implanted into rabbit knee osteochondral defects.
  • Repaired tissues were evaluated macroscopically, histologically, and immunohistologically at 4, 8, and 12 weeks.

    • Main Results:

    • Grafts with BMP-7 gene-modified cells demonstrated consistent complete or near-complete bone and articular cartilage regeneration at 8 and 12 weeks.
    • Control groups showed poor repair outcomes.
    • The combined gene therapy and tissue engineering approach led to successful cartilage regeneration.

    Conclusions:

    • Combined gene therapy and tissue engineering effectively regenerate articular cartilage and bone in an osteochondral defect model.
    • This approach represents a pioneering method for articular cartilage regeneration.
    • The findings suggest a promising therapeutic strategy for joint cartilage repair.