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Related Experiment Video

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Intravital Imaging of Intraepithelial Lymphocytes in Murine Small Intestine

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Published on: June 24, 2019

Interleukin 15 mediates epithelial changes in celiac disease.

L Maiuri¹, C Ciacci, S Auricchio

¹Department of Pediatrics, University "Federico II," Naples, Italy.

Gastroenterology

|October 21, 2000

Summary

Interleukin-15 (IL-15) drives epithelial changes in celiac disease, including increased cell proliferation and apoptosis. Neutralizing IL-15 reduces gliadin-induced damage, highlighting its role in celiac pathology.

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Area of Science:

Gastroenterology
Immunology
Cell Biology

Background:

Celiac disease is characterized by villous atrophy and crypt proliferation.
Epithelial changes in celiac disease may be influenced by cytokines sharing receptor chains.

Purpose of the Study:

To investigate the role of interleukin-15 (IL-15) and other cytokines in celiac disease epithelial modifications.
To determine if IL-15 influences enterocyte proliferation, apoptosis, and expression of specific markers.

Main Methods:

Duodenal biopsy specimens from celiac patients and controls were cultured in vitro.
Cytokines (IL-15, IL-7, IL-4, IL-2) and gliadin were added to assess their effects on epithelial markers (Ki67, FAS, TFR) and apoptosis.
Immunohistochemistry and TUNEL assay were used to detect epithelial changes and IL-15-positive cells.

Main Results:

IL-15 significantly induced enterocyte proliferation (Ki67), transferrin receptor (TFR) expression, FAS expression, and apoptosis in celiac disease specimens.
Anti-IL-15 antibodies neutralized gliadin-induced effects.
IL-15-positive cells were significantly increased in untreated celiac disease.

Conclusions:

IL-15 plays a critical role in modulating epithelial changes observed in celiac disease.
IL-15 has an unexpected and significant role in the pathological manifestations of celiac disease.