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Effect of enterocin CRL35 on Listeria monocytogenes cell membrane
C J Minahk1, M E Farías, F Sesma
1Departamento de Bioquimica de la Nutricion, Instituto Superior de Investigaciones Biológicas, Instituto de Quimica Biologica, San Miguel de Tucuman, Argentina.
FEMS Microbiology Letters
|October 21, 2000
Abstract:
The antimicrobial peptide Enterocin CRL35, a class II bacteriocin, produces at high concentrations (8 microg ml(-1)) localized holes in the wall and cellular membrane of Listeria monocytogenes, reflected in the efflux of macromolecules such as proteins and other ultraviolet-absorbing materials. At lower concentrations (0.5 microg ml(-1)), neither ultra structural changes nor macromolecules efflux were observed, however potassium and phosphate ions were released, dissipating the proton motive force. As a result the bacteria were killed.