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Related Experiment Videos

Development of a Simple Cell Invasion Assay System.

S Yamakawa1, Y Furuyama, N Oku

  • 1Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan.

Biological & Pharmaceutical Bulletin
|October 21, 2000
PubMed
Summary

This study introduces a simple fluorescence blocking micropore membrane (FBM) method for quantifying cell invasion. This technique streamlines cancer metastasis and angiogenesis research by simplifying cell invasion assays.

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Area of Science:

  • Cell biology
  • Biotechnology
  • Cancer research

Background:

  • Cell invasion assays are crucial for understanding cancer metastasis, inflammation, and angiogenesis.
  • Traditional methods often involve complex steps like cell fixation, staining, and removal of non-invaded cells.

Purpose of the Study:

  • To develop a simplified and accurate method for quantifying cell invasion.
  • To establish a novel assay using a fluorescence blocking micropore membrane (FBM).

Main Methods:

  • A culture insert with an 8-micropore FBM, precoated with Matrigel, was utilized.
  • Fluorescence-labeled cells were added to the insert and incubated.
  • The number of invaded cells was quantified by examining the FBM under a fluorescence microscope.

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Main Results:

  • The developed method allows for straightforward quantification of cell invasion.
  • It eliminates the need for cell fixation, staining, and removal of non-invaded cells, simplifying the process.

Conclusions:

  • This FBM-based method offers an easy and accurate approach to cell invasion assays.
  • It is a valuable tool for studying biological events like cancer metastasis and angiogenesis.