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Related Experiment Videos

Modulation of corneal endothelial hydration control mechanisms by Rolipram.

C G Wigham1, H C Turner, J Swan

  • 1Department of Optometry and Vision Sciences, Cardiff University, UK. Wighamcg@cardiff.ac.uk

Pflugers Archiv : European Journal of Physiology
|October 21, 2000
PubMed
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Rolipram, a cAMP phosphodiesterase inhibitor, increases intracellular cAMP levels, leading to corneal thinning and altered endothelial transport. This suggests cyclic AMP regulates both endothelial pump activity and permeability, maintaining corneal hydration.

Area of Science:

  • Ophthalmology
  • Cell Physiology
  • Corneal Biology

Background:

  • Corneal hydration is crucial for vision and is regulated by endothelial bicarbonate (HCO3-) transport.
  • Intracellular cyclic adenosine monophosphate (cAMP) influences this transport, but its precise site of action is unknown.

Purpose of the Study:

  • To investigate the effects of Rolipram, a selective cAMP phosphodiesterase inhibitor, on corneal endothelial physiology.
  • To determine the role of intracellular cAMP in regulating corneal hydration and endothelial transport mechanisms.

Main Methods:

  • Addition of 0.1 mM Rolipram to corneal tissue.
  • Measurement of intracellular cAMP and cyclic guanosine monophosphate (cGMP) levels.
  • Assessment of corneal thickness, trans-endothelial potential difference, short-circuit current, resistance, membrane potential, and intracellular Na+ concentration.

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Main Results:

  • Rolipram significantly increased intracellular cAMP levels threefold without affecting cGMP.
  • Corneal thinning, increased trans-endothelial potential difference, short-circuit current, and resistance were observed transiently.
  • Rolipram induced membrane hyperpolarization and decreased intracellular Na+ concentration, indicating enhanced Na+ extrusion.

Conclusions:

  • Rolipram-induced cAMP increase activates basolateral Na+/K+-ATPase activity, enhancing net HCO3- transport.
  • Reduced endothelial permeability and increased pump activity contribute to corneal stromal thinning.
  • Cyclic AMP may physiologically regulate corneal hydration by modulating both endothelial pump function and permeability.