Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Separable whorl-specific expression and negative regulation by enhancer elements within the AGAMOUS second intron.

M K Deyholos1, L E Sieburth

  • 1Biology Department, McGill University, Montreal, Quebec, Canada H3A 1B1.

The Plant Cell
|October 21, 2000
PubMed
Summary

The AGAMOUS (AG) gene

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Amaranth Genome: Genome, Transcriptome, and Physical Map Assembly.

The plant genome·2016
Same author

The Evolution of HD2 Proteins in Green Plants.

Trends in plant science·2016
Same author

High-density microarrays for gene expression analysis.

Cytometry·2001
Same author

The SCARFACE gene is required for cotyledon and leaf vein patterning.

Development (Cambridge, England)·2000
Same author

Auxin is required for leaf vein pattern in Arabidopsis.

Plant physiology·1999
Same author

Non-autonomy of AGAMOUS function in flower development: use of a Cre/loxP method for mosaic analysis in Arabidopsis.

Development (Cambridge, England)·1998

Area of Science:

  • Plant molecular biology
  • Developmental genetics
  • Arabidopsis research

Background:

  • The AGAMOUS (AG) gene is crucial for floral organ development in Arabidopsis.
  • Understanding the regulatory mechanisms of AG gene expression is key to floral development studies.

Purpose of the Study:

  • To investigate the regulatory elements controlling AG gene expression during early flower development.
  • To elucidate the roles of APETALA2 (AP2) and LEUNIG (LUG) in modulating AG expression patterns.

Main Methods:

  • Analysis of 19 AG::reporter gene constructs to identify expression patterns.
  • Examination of beta-glucuronidase staining in Arabidopsis mutants for ap2 and lug.

Main Results:

  • Three distinct AG expression patterns were identified: normal, stamen-specific, and carpel-specific.

Related Experiment Videos

  • Stamen-specific expression is LUG-dependent and AP2-independent.
  • Carpel-specific expression is AP2-dependent and LUG-independent.

    • Conclusions:

    • AG gene expression in floral organ development is regulated by independent positive and negative controls for different whorls.
    • AP2 and LUG act as key regulators, with distinct roles in controlling AG expression in stamens and carpels.