Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adrenocortical function in a new world primate, the marmoset monkey, Callithrix jacchus.

B J Whitehouse1, D R Abayasekara

  • 1Endocrinology and Reproduction Research Group, King's College London, Guy's Campus, London, SE1 1UL.

General and Comparative Endocrinology
|October 24, 2000
PubMed
Summary

Marmoset monkeys exhibit high plasma cortisol levels, yet their adrenal cells do not respond to ACTH stimulation in vitro. This suggests a reduced adrenal ACTH receptor function, not a general cell defect, in Callithrix jacchus.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of protein kinase C in the inhibitory action of trophoblast interferons on expression of the oxytocin receptor in sheep endometrium.

Endocrine·2010
Same author

Direct regulation of insulin secretion by angiotensin II in human islets of Langerhans.

Diabetologia·2006
Same author

ACTH stimulates insulin secretion from MIN6 cells and primary mouse and human islets of Langerhans.

The Journal of endocrinology·2004
Same author

Interdependence of steroidogenesis and shape changes in Y1 adrenocortical cells: studies with inhibitors of phosphoprotein phosphatases.

The Journal of endocrinology·2002
Same author

Relationship between the production of prostaglandins and progesterone by luteinizing human granulosa cells.

The Journal of endocrinology·2001
Same author

Human granulosa-lutein cells express functional EP1 and EP2 prostaglandin receptors.

Biochemical and biophysical research communications·2001

Area of Science:

  • Endocrinology
  • Primate Physiology
  • Adrenal Gland Function

Background:

  • The adrenal cortex regulates stress response and metabolism.
  • New World primates, including marmosets (Callithrix jacchus), are known for potential glucocorticoid resistance.
  • Understanding adrenal function in marmosets is crucial for comparative endocrinology.

Purpose of the Study:

  • To investigate the functional capacity of the marmoset adrenal cortex in response to various stimuli.
  • To determine the mechanism behind the apparent glucocorticoid resistance in Callithrix jacchus.
  • To assess the role of adrenocorticotropic hormone (ACTH) and other signaling pathways in marmoset adrenal cell function.

Main Methods:

  • Dispersed adrenal cells from male and female marmosets were prepared using collagenase digestion.

Related Experiment Videos

  • Cells were incubated with adrenocorticotropic hormone (ACTH), angiotensin II, dibutyryl cyclic AMP (dbcAMP), and forskolin (FSK).
  • Plasma cortisol and aldosterone levels, as well as cyclic AMP production, were measured.

    • Main Results:

    • Plasma cortisol levels in marmosets were significantly higher (10-20 fold) than in Old World primates and humans.
    • Adrenal cell preparations showed no significant response to ACTH (0.1-1000 nM).
    • Responses to angiotensin II, dbcAMP, and FSK indicated intact post-receptor signaling for aldosterone and cortisol production, respectively.

    Conclusions:

    • The lack of in vitro ACTH response in marmoset adrenal cells is not due to general cell failure or post-receptor defects.
    • Results suggest a reduced number or affinity of adrenal ACTH receptors in Callithrix jacchus.
    • Elevated in vivo plasma cortisol levels are likely maintained by a high basal production rate, despite impaired ACTH signaling.