Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Classical swine fever virus: a second ring test to evaluate RT-PCR detection methods.

D J Paton1, A McGoldrick, E Bensaude

  • 1Department of Virology, Veterinary Laboratories Agency, Central Veterinary Laboratory - Weybridge, Woodham Lane, New Haw, Surrey KT15 3NB, Addlestone, UK. dpaton.cvl.wood@gtnet.gov.nk

Veterinary Microbiology
|October 24, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The utility of the Sports Concussion Assessment Tool in hospitalized traumatic brain injury patients.

Journal of concussion·2026
Same author

Are the knowledge, tools and resources to control foot and mouth disease available?

Revue scientifique et technique (International Office of Epizootics)·2024
Same author

[Expectations of patients and relatives for modern hospital care in geriatric psychiatry].

Zeitschrift fur Gerontologie und Geriatrie·2021
Same author

Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease.

Revue scientifique et technique (International Office of Epizootics)·2021
Same author

Common mental disorders among Irish jockeys: prevalence and risk factors.

The Physician and sportsmedicine·2020
Same author

Estimating the protection afforded by foot-and-mouth disease vaccines in the laboratory.

Vaccine·2019
Same journal

A divalent nanoparticle vaccine confers robust protective immunity against serotype O FMDV and SVA in pigs.

Veterinary microbiology·2026
Same journal

A Lactobacillus plantarum vaccine candidate with dendritic cell targeting and lysosome escape properities provides efficient protection against necrotic enteritis in broiler chickens.

Veterinary microbiology·2026
Same journal

Tracheal transcriptional response to challenge with virulent Mycoplasma gallisepticum in chickens spray vaccinated with the Vaxsafe MG304 live-attenuated vaccine.

Veterinary microbiology·2026
Same journal

Intermittent shedding dynamics of EHV-1 across biological matrices during a natural outbreak linked to international equestrian events.

Veterinary microbiology·2026
Same journal

Development of a replication-restricted, esterase-deficient influenza D virus as a live vaccine candidate.

Veterinary microbiology·2026
Same journal

Co-infection with CAV facilitates ALV-K replication and induces severe immunosuppression in chickens.

Veterinary microbiology·2026
See all related articles

Standardized RT-PCR protocols improved classical swine fever virus (CSFV) detection accuracy in a six-lab study. Consistent sensitivity was achieved, with three labs reporting no false positives, highlighting the importance of controls and proficiency testing.

Area of Science:

  • Veterinary Virology
  • Molecular Diagnostics
  • Food Animal Health

Background:

  • Classical swine fever virus (CSFV) poses a significant threat to swine populations globally.
  • Accurate and reliable diagnostic tests are crucial for controlling CSFV outbreaks.
  • Previous studies indicated variability in RT-PCR test performance for CSFV detection.

Purpose of the Study:

  • To compare the sensitivity and specificity of RT-PCR tests for CSFV detection across multiple laboratories.
  • To evaluate the impact of standardized protocols on RT-PCR test performance.
  • To establish minimum requirements for diagnostic controls and proficiency testing.

Main Methods:

  • Six laboratories participated in a ring trial using coded samples of cDNA, blood, and serum.

Related Experiment Videos

  • Samples were derived from various pestiviruses and CSFV-infected or virus-free pigs.
  • Two RT-PCR methods were evaluated: one targeting the 5'-non-coding region (RT-nested PCR) and another targeting the NS5B gene (non-nested RT-PCR).

    • Main Results:

    • Standardization of RT-PCR protocols led to more consistent test sensitivity across laboratories.
    • Three laboratories successfully avoided significant false positive results.
    • Laboratories could identify inadequate test specificity based on control sample results.

    Conclusions:

    • Standardized protocols enhance the reliability of RT-PCR for CSFV detection.
    • Adequate controls and regular proficiency testing are essential for ensuring diagnostic accuracy.
    • The study proposes minimum requirements for implementing effective CSFV diagnostic assays.