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Related Experiment Videos

Ethanol utilization by sulfate-reducing bacteria: an experimental and modeling study.

S Nagpal1, S Chuichulcherm, A Livingston

  • 1Department of Chemical Engineering and Chemical Technology, Imperial College, London, SW7 2BY, United Kingdom.

Biotechnology and Bioengineering
|October 24, 2000
PubMed
Summary

Sulfate-reducing bacteria showed slower growth and lower yield with ethanol compared to lactate. Ethanol conversion produced acetate, not carbon dioxide, with pH variations analyzed in a new mathematical model.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Environmental Science

Background:

  • Sulfate-reducing bacteria (SRB) are crucial in biogeochemical cycles.
  • Understanding SRB metabolism is key for applications like wastewater treatment and bioremediation.
  • Ethanol as a carbon source for SRB presents unique metabolic pathways and challenges.

Purpose of the Study:

  • To investigate the stoichiometry and kinetics of sulfate reduction by Desulfovibrio desulfuricans using ethanol.
  • To develop and validate a mathematical model for SRB growth on ethanol.
  • To analyze pH variations and hydrogen sulfide dynamics during ethanol metabolism.

Main Methods:

  • Cultivation of a mixed SRB culture including Desulfovibrio desulfuricans.
  • Experimental determination of growth yield and reaction kinetics with ethanol and lactate.

Related Experiment Videos

  • Development of a mathematical model incorporating ethanol metabolism and pH changes.
  • Simulation of growth experiments and analysis of hydrogen sulfide removal via nitrogen sparging.

    • Main Results:

    • Lower growth yield and slower kinetics were observed with ethanol compared to lactate.
    • Ethanol was primarily converted to acetate, with minimal carbon dioxide production.
    • The developed mathematical model accurately simulated experimental observations.
    • pH variations and hydrogen sulfide dynamics were successfully analyzed.

    Conclusions:

    • Ethanol is a viable but less efficient substrate for Desulfovibrio desulfuricans compared to lactate.
    • The new mathematical model provides a valuable tool for predicting SRB behavior with ethanol.
    • The study elucidates the specific metabolic fate of ethanol and its impact on the culture environment.