Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Localised MPF regulation in eggs.

C Beckhelling1, D Pérez-Mongiovi, E Houliston

  • 1Unité de Biologie du Développement, UMR 7009 CNRS, Université Paris VI, Villefranche-sur-mer, France.

Biology of the Cell
|October 24, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Oral Microbiome in Health and Its Implication in Oral and Systemic Diseases.

Advances in applied microbiology·2016
Same author

The pitx2 homeobox protein is required early for endoderm formation and nodal signaling. .

Developmental biology·2001
Same author

Nuclei and microtubule asters stimulate maturation/M phase promoting factor (MPF) activation in Xenopus eggs and egg cytoplasmic extracts.

The Journal of cell biology·2000
Same author

Local inhibition of cortical rotation in Xenopus eggs by an anti-KRP antibody.

Developmental biology·2000
Same author

Timing of calcium and protein synthesis requirements for the first mitotic cell cycle in fertilised Xenopus eggs.

Journal of cell science·1999
Same author

Organisation of Xenopus oocyte and egg cortices.

Microscopy research and technique·1999
Same journal

Oxidative Stress and Nrf2 Signaling Role in Oral Squamous Cell Carcinoma: A Double-Edged Sword.

Biology of the cell·2026
Same journal

Wnt Stimulation and Inhibition in the Development and Phenotype of Patient-Derived Gallbladder Organoids.

Biology of the cell·2026
Same journal

From Genomic Alterations to Functional Tumor Biology: Integrating Organoids and Organ-on-a-Chip in Colorectal Cancer.

Biology of the cell·2026
Same journal

Delta-Cell Area is Unchanged in Human Pregnancy: Evidence From Immunohistochemistry.

Biology of the cell·2026
Same journal

Rearrangement of the Cell Chaperone Machinery in Human Fibrosarcoma HT1080 Cells With the Knocked-Out HSP90AA1 Gene Encoding Hsp90α.

Biology of the cell·2026
Same journal

STAT Signaling and Its Anti-Apoptotic Effects in Dehydrated Xenopus laevis.

Biology of the cell·2026
See all related articles

M-phase promoting factor (MPF) activation and inactivation are locally controlled within cells, involving calcium signals and molecular regulators near the nucleus and spindle. This localized regulation ensures proper cell division timing.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • M-phase promoting factor (MPF) is the key regulator of cell mitosis.
  • Understanding MPF regulation is crucial for comprehending cell cycle control.

Purpose of the Study:

  • To review evidence for localized regulation of MPF activation and inactivation.
  • To explore the molecular mechanisms underlying MPF's spatial control during mitosis.

Main Methods:

  • Analysis of surface contraction waves (SCWs) as indicators of MPF activity.
  • Direct measurement of MPF activity in cell fragments.
  • Examination of MPF regulators and calcium signaling in various cell types.

Main Results:

Related Experiment Videos

  • MPF activation is initiated locally, often near the nucleus and microtubules.
  • The perinuclear/centrosomal region accumulates MPF and its regulators.
  • Calcium transients are essential for both MPF activation and inactivation, coordinating with localized regulators and spindle structures.

    • Conclusions:

    • MPF regulation is spatially controlled, with localized calcium signals and molecular machinery orchestrating mitotic entry and exit.
    • The interplay between calcium signaling, MPF regulators, and cellular structures ensures precise cell division.