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Retrovirus vector containing wild type p53 gene and its effect on human glioma cells
J Hlavatý1, S Tyukosova, J Bies
1Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Neoplasma
|October 24, 2000
Abstract:
A retroviral vector containing wild-type p53 tumor suppressor gene (wt-p53) under the control of viral LTR sequences was constructed and transfected into packaging cell line GP+envAm12. Virus producing single cell clone GP+envAm12/ p53clC8 (8 x 10(5) cfu/ml, determined on NIH 3T3 cells) was isolated and used to transfer wt-p53 gene into human glioma cell lines in vitro. Decreased viability in p53-infected cells as compared to uninfected or empty virus infected cells was observed.