Related Experiment Video
Updated: Sep 3, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
The two slow refolding processes of creatine kinase are catalyzed by cyclophilin
1National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, China.
Abstract:
A burst phase occurs in the refolding kinetics of guanidine-denatured creatine kinase due to formation of an intermediate within the mixing dead time, with further refolding to the native state after the burst phase along a path following biphasic kinetics. In the presence of cyclophilin, the refolding rates of the two slow processes are accelerated and the values are proportional to the cyclophilin concentration. The activity of cyclophilin in accelerating the slow refolding processes of creatine kinase is totally inhibited by cyclosporin A, indicating that the cis-trans isomerization of the peptidyl-prolyl bonds is involved in the two slow refolding processes.
Related Concept Videos
Protein Folding
Molecular Chaperones and Protein Folding
The...
ATP Synthase: Mechanism
Energy to Drive Translocation
Generally, polypeptides are unfolded by two distinct...
Anaphase Promoting Complex
Protein Folding
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...

