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A comparative study on the disintegration of filamentous fungi
J Taubert1, U Krings, R G Berger
1Institut für Lebensmittelchemie, Universität Hannover, Wunstorfer Strasse 14, 30453, Hannover, Germany.
Abstract:
Different methods for cell disintegration were tested for their efficacy on filamentous fungi, including percussion grinding, homogenization using an Ultra-Turrax, chemical treatment and lyophylization. The release of protein from Ganoderma applanatum and Pycnoporus cinnabarinus and the activity of cytoplasmatic glucose-6-phosphate dehydrogenase in the crude extracts were monitored to determine the efficiency of each disintegration technique used. Fungal cells proved to be particularly resistant towards some disintegration methods commonly used for yeasts and bacteria. Best results were obtained using a percussion grinder, if necessary, in combination with an Ultra-Turrax pretreatment.
Insights
Percussion grinding is the most effective method for fungal cell disintegration, outperforming common techniques. This method, sometimes combined with Ultra-Turrax pretreatment, efficiently releases proteins and enzymes from filamentous fungi.
Area of Science:
- Mycology
- Biochemistry
- Biotechnology
Background:
- Filamentous fungi are crucial in various biotechnological applications.
- Efficiently releasing intracellular components from fungal cells is essential for downstream applications.
- Standard cell disintegration methods for yeasts and bacteria are often ineffective for fungi.
Purpose of the Study:
- To evaluate the efficacy of different cell disintegration methods for filamentous fungi.
- To identify the optimal method for protein and enzyme release from fungal cells.
- To compare the effectiveness of percussion grinding, Ultra-Turrax homogenization, chemical treatment, and lyophilization.
Main Methods:
- Tested percussion grinding, Ultra-Turrax homogenization, chemical treatment, and lyophilization on Ganoderma applanatum and Pycnoporus cinnabarinus.
- Monitored protein release as a measure of cell disruption.
- Assayed cytoplasmic glucose-6-phosphate dehydrogenase activity in crude extracts to assess enzyme release.
Main Results:
- Filamentous fungal cells exhibited significant resistance to common disintegration methods.
- Percussion grinding demonstrated superior efficacy in disrupting fungal cells.
- Combining percussion grinding with Ultra-Turrax pretreatment yielded the best results for protein and enzyme release.
Conclusions:
- Percussion grinding is a highly effective method for the disintegration of filamentous fungi.
- The choice of disintegration method is critical for efficient extraction of intracellular fungal components.
- Optimized cell disruption protocols are necessary for advancing fungal biotechnology.