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Epitope tagging: general method for tracking recombinant proteins.

C E Fritze1, T R Anderson

  • 1Covance Research Products, Inc., Richmond, California 94804-4609, USA.

Methods in Enzymology
|October 25, 2000
PubMed
Summary
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Epitope tagging is a versatile molecular biology technique with broad applications. Its diverse tag systems offer experimental flexibility, ensuring its continued value for researchers.

Area of Science:

  • Molecular Biology
  • Biotechnology

Background:

  • Epitope tagging is an established experimental strategy.
  • A wide array of epitope tag systems are currently available.

Purpose of the Study:

  • To highlight the broad applicability and continued relevance of epitope tagging in molecular biology.

Main Methods:

  • Review of existing epitope tag systems.
  • Assessment of their experimental utility and adaptability.

Main Results:

  • Epitope tagging demonstrates widespread applicability across various experimental systems.
  • The variety of available tag systems provides adaptable attributes for diverse research needs.

Conclusions:

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  • Epitope tagging remains a valuable and indispensable tool for molecular biologists.
  • Its versatility ensures its ongoing utility in future research endeavors.