Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural and image analysis of a crystalline layer from dipteran eggshell.

L H Margaritis, W H Petri, A R Wyman

    Cell Biology International Reports
    |January 1, 1979
    PubMed
    Summary

    Researchers identified a crystalline intermediate chorionic layer (ICL) in Drosophila melanogaster eggshells. This layer exhibits a P2 planar periodicity, revealing structural details of insect egg development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effects of pre- and postnatal exposure to 1880-1900MHz DECT base radiation on development in the rat.

    Reproductive toxicology (Elmsford, N.Y.)·2016
    Same author

    Reply to drs. R. Glaser and ch. Bornkessel.

    Pathophysiology : the official journal of the International Society for Pathophysiology·2011
    Same author

    Short-term memory in mice is affected by mobile phone radiation.

    Pathophysiology : the official journal of the International Society for Pathophysiology·2010
    Same author

    Reply to drs. R. Glaser and ch. Bornkessel.

    Pathophysiology : the official journal of the International Society for Pathophysiology·2010
    Same author

    Whole body exposure with GSM 900MHz affects spatial memory in mice.

    Pathophysiology : the official journal of the International Society for Pathophysiology·2009
    Same author

    Implantation of MNNG crystals into a Triturus intact limb affects mitotic and labeling indices, regeneration rate, and morphogenesis in the contralateral, regenerating limb.

    Teratogenesis, carcinogenesis, and mutagenesis·2002

    Area of Science:

    • * Insect reproductive biology
    • * Developmental biology
    • * Structural biology

    Background:

    • * The eggshell of the dipteran Drosophila melanogaster is a complex structure crucial for embryonic development.
    • * Previous research has focused on the outer layers, with less known about the intermediate structures.

    Purpose of the Study:

    • * To identify and characterize a crystalline layer within the Drosophila melanogaster eggshell.
    • * To elucidate the structural organization and periodicity of this newly identified layer.

    Main Methods:

    • * Isolation and negative staining of the intermediate chorionic layer (ICL).
    • * Analysis of whole mount views using optical diffraction and filtering techniques.

    Main Results:

    Related Experiment Videos

    • * Identification of a 400Å thick crystalline intermediate chorionic layer (ICL) composed of eight 50Å sublayers.
    • * The ICL is situated between the vitelline membrane and the endochorion.
    • * Negative staining revealed a P2 planar periodicity with 1st (100Å), 2nd, 3rd, and 4th order reflections.

    Conclusions:

    • * A distinct crystalline layer with ordered sublayers exists within the Drosophila melanogaster eggshell.
    • * The observed periodicity provides insights into the structural organization and potential formation mechanisms of the eggshell.