New techniques for the preparation of uniform layers of nuclear emulsions for use in micro-autoradiography

A procedure is described whereby liquid emulsion can be used to coat glass coverslips to produce films of emulsion suitable for quantitative evaluation of grain density in micro-autoradiographs. Any thickness of emulsion layer can be produced from 3 mum upwards, depending on the volume and dilution of the emulsion dispensed. The uniformity and reproducibility of the films have been tested by observing light transmission of undeveloped preparations using a microscope photometer.