Separation and identification of volatile components in the fermentation broth of Trichoderma atroviride by
A Keszler1, E Forgács, L Kótai
1Institute of Chemistry, Chemical Research Center, Hungarian Academy of Sciences, Budapest. agi@cric.chemres.hu
Abstract:
A preseparated fermentation broth of Trichoderma atroviride strain 11 is analyzed by gas chromatography followed by mass-spectral detection using a Finnigan MAT GCQ apparatus. After preseparation in a C18 and a silica gel column, nineteen pyrone and dioxolane derivatives and two aliphatic esters are obtained, respectively. Among these, the four dioxolane derivatives have not been identified previously. The main component is found to be 5,5'-dimethyl-2H-pyran-2-on. The relative standard deviation for the determination of the retention time and the peak area (measured in ion counts) is 0.1% and 4.5%, respectively.
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