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Related Experiment Videos

Minimum detectable level of Salmonellae using a binomial-based bacterial ice nucleation detection assay (BIND).

P Irwin1, A Gehring, S I Tu

  • 1US Department of Agriculture, Eastern Regional Research Center, Microbial Biophysics & Biochemistry Laboratory, Wyndmoor, PA 19038, USA.

Journal of AOAC International
|October 26, 2000
PubMed
Summary

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A modified bacterial ice nucleation detection (BIND) assay offers rapid Salmonella detection. This sensitive method identifies species like S. enteritidis with high accuracy, aiding in food safety and diagnostics.

Area of Science:

  • Microbiology
  • Food Safety
  • Biotechnology

Background:

  • Salmonella species pose significant public health risks.
  • Accurate and rapid detection methods are crucial for controlling Salmonella outbreaks.
  • Existing detection methods can be time-consuming or lack sensitivity.

Purpose of the Study:

  • To develop and validate a modified bacterial ice nucleation detection (BIND) assay for Salmonella species.
  • To assess the sensitivity and quantitative capabilities of the BIND assay.
  • To evaluate the integration of immunomagnetic bead separation with the BIND assay for enhanced detection.

Main Methods:

  • Utilized a modified bacterial ice nucleation detection (BIND) assay involving bacteriophage infection of Salmonella.
  • Incorporated ice nucleation protein (INP) synthesis and incorporation into bacterial outer membranes.

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  • Employed supercooling and a phase-sensitive dye for detection, coupled with a probability-based protocol modification.
  • Integrated Salmonella-specific immunomagnetic bead separation technology.

    • Main Results:

    • Achieved rapid and sensitive detection of Salmonella species, including S. enteritidis, S. typhimurium DT104, and S. abaetetuba.
    • Established minimum detectable levels (MDLs) as low as 2.0 +/- 0.3 S. enteritidis cells/mL in buffer within approximately 3 hours.
    • Demonstrated an MDL of approximately 4.5 S. enteritidis cells/mL with 56% capture efficiency when using immunomagnetic bead separation.

    Conclusions:

    • The modified BIND assay provides a rapid, sensitive, and quantitative method for detecting Salmonella species.
    • The assay's performance is suitable for various Salmonella serovars.
    • Combining BIND assay with immunomagnetic separation further enhances detection capabilities, offering a promising tool for food safety and clinical diagnostics.