Related Experiment Video
Updated: Sep 2, 2026

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry.
Published on: September 1, 2017
Determination of selenium in serum by FI-HG-AAS and calculation of dietary intake
F Li1, E Rossipal, D Micetic-Turk
1Department of Applied Chemistry, Nanjing University of Chemical Technology, People's Republic of China.
Abstract:
A method was developed for the determination of selenium concentration in serum by flow injection-hydride generation-atomic absorption spectrometry (FI-HG-AAS) following microwave digestion of serum samples and reduction of selenate to selenite. The detection limit of the method was 0.3 microg Se/L and the characteristic concentration, corresponding to the 0.0044 absorbance signal, was 0.12 microg Se/L. The results from the analysis of two Seronorm standard reference materials showed good agreement with the certified values. The method was then used to analyze selenium in sera of Austrian and Slovenian people for the calculation of dietary intakes. The selenium concentrations in sera of mothers at delivery, their neonates, and the male and female adults were 71+/-14, 42+/-6, 75+/-21, and 65+/-16 microg/L for the Austrians and 62+/-15, 34+/-7, 70+/-12, and 66+/-15 microg/L for the Slovenians. The dietary intakes of selenium of the mothers and the male and the female adults were calculated as 52, 37, and 46 microg/d for the Austrians and 45, 38, and 32 microg/d for the Slovenians.
Related Concept Videos
Atomic Absorption Spectroscopy: Overview
When irradiated by EMR of a particular wavelength, these...
Atomic Absorption Spectroscopy: Lab
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Serum Laboratory Studies, Stool Test, Breath Test

