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Updated: Jul 29, 2026

A Method for Labeling Vasculature in Embryonic Mice
Published on: October 7, 2011
Role of SCL/Tal-1, GATA, and ets transcription factor binding sites for the regulation of flk-1 expression during
A Kappel1, T M Schlaeger, I Flamme
1Max-Planck-Institute for Physiological and Clinical Research, Bad Nauheim, Germany.
Abstract:
The receptor tyrosine kinase Flk-1 is essential for embryonic blood vessel development and for tumor angiogenesis. To identify upstream transcriptional regulators of Flk-1, the gene regulatory elements that mediate endothelium-specific expression in mouse embryos were characterized. By mutational analysis, binding sites for SCL/Tal-1, GATA, and Ets transcription factors located in the Flk-1 enhancer were identified as critical elements for the endothelium-specific Flk-1 gene expression in transgenic mice. c-Ets1, a transcription factor that is coexpressed with Flk-1 during embryonic development and tumor angiogenesis, activated the Flk-1 promoter via 2 binding sites. One of these sites was required for Flk-1 promoter function in the embryonic vasculature. These results provide the first evidence that SCL/Tal-1, GATA, and Ets transcription factors act upstream of Flk-1 in a combinatorial fashion to determine embryonic blood vessel formation and are key regulators not only of the hematopoietic program, but also of vascular development.
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