Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reverse vaccinology.

R Rappuoli1

  • 1IRIS, Chiron S.p.A., Via Fiorentina 1, 53100, Siena, Italy. Rino_Rappuoli@biocine.it

Current Opinion in Microbiology
|October 26, 2000
PubMed
Summary
This summary is machine-generated.

Genomic approaches enable reverse vaccinology, a new method for vaccine development. This in silico process bypasses traditional cultivation, allowing the creation of vaccines against difficult pathogens.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbes without borders: uniting societies for climate action.

mBio·2025
Same author

Microbes without borders: uniting societies for climate action.

FEMS microbiology ecology·2025
Same author

Microbial solutions for climate change require global partnership.

mBio·2025
Same author

Development and Characterization of a 13-Plex Binding Assay to Detect <i>Shigella</i> Antibodies in Human Samples.

Open forum infectious diseases·2024
Same author

Immunological fingerprint of 4CMenB recombinant antigens via protein microarray reveals key immunosignatures correlating with bactericidal activity.

Nature communications·2020
Same author

Farewell Stan Stanley Falkow: 1934-2018.

Environmental microbiology·2018
Same journal

The role of the antimicrobial peptide nisin as a clean label food preservative.

Current opinion in microbiology·2026
Same journal

From coarse-grained metabolic rules to fine-grained control of microbial communities.

Current opinion in microbiology·2026
Same journal

Progress in engineered bacterial cancer therapies.

Current opinion in microbiology·2026
Same journal

Constraints on adaptive loss-of-function mutations during microbial metabolic interactions.

Current opinion in microbiology·2026
Same journal

Discovery of novel antimicrobials within microbiomes.

Current opinion in microbiology·2026
Same journal

Beyond the protein lattice: bacterial S-layer glycans - from structure to functional frontier.

Current opinion in microbiology·2026
See all related articles

Area of Science:

  • * Infectious disease research
  • * Vaccine development
  • * Genomics and immunology

Background:

  • * Traditional vaccine development relies on biochemical, serological, and microbiological methods to identify pathogen components.
  • * These methods are time-consuming and limited by the inability to cultivate certain pathogens in vitro or by antigen sequence variability.
  • * Limitations hinder the development of vaccines against numerous pathogens.

Purpose of the Study:

  • * To introduce and define reverse vaccinology as a novel approach to vaccine development.
  • * To highlight the advantages of genomic approaches over traditional methods.
  • * To emphasize the potential of reverse vaccinology in creating vaccines previously considered impossible.

Main Methods:

Related Experiment Videos

  • * Utilizes genomic information for in silico antigen prediction.
  • * Bypasses the need for in vitro pathogen cultivation.
  • * Identifies all potential antigens, irrespective of abundance or immunogenicity during infection.

    • Main Results:

    • * Genomic approaches allow comprehensive antigen prediction.
    • * Enables vaccine development against pathogens that cannot be cultured.
    • * Facilitates the use of non-conventional antigens and immune system pathways.

    Conclusions:

    • * Reverse vaccinology represents a paradigm shift in vaccine discovery.
    • * This in silico-driven method accelerates vaccine development and broadens its scope.
    • * It promises to make vaccines for previously intractable diseases a reality.