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Purification of proteins using UltraMacro spin columns or ProSorb sample preparation cartridges for amino acid

L Zhang1, N Denslow

  • 1Protein Chemistry Core Laboratory, University of Florida, Gainesville, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 29, 2000
PubMed
Abstract

No abstract available in PubMed .

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
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