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[Cloning and sequencing of the maize ribosome-inactivating protein gene]
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|October 29, 2000
Abstract:
A gene encoding maize Ribosome-inactivating protein was amplified by means of PCR using mRNA of maize leaves as a template, and cloned into pUC19 vector. The amplified DNA sequence has been determined, which consists of 828 bp and encodes 275 amino acid residues. Comparison with previously reported sequence shows 98.4% homologies in nucleotide sequence and 97.4% in amino acid sequence, respectively.
Insights
Researchers amplified and cloned a maize Ribosome-inactivating protein gene. The determined sequence shows high homology to previously reported sequences, aiding in understanding plant defense mechanisms.
Area of Science:
- Molecular Biology
- Plant Science
- Genetics
Context:
- Ribosome-inactivating proteins (RIPs) are plant defense proteins.
- Understanding the genetic basis of RIPs is crucial for crop improvement.
Purpose:
- To amplify and clone the gene encoding maize Ribosome-inactivating protein.
- To determine the nucleotide sequence of the amplified gene.
- To compare the sequence with previously reported sequences.
Summary:
- A gene for maize Ribosome-inactivating protein was amplified using PCR from maize leaf mRNA.
- The gene was cloned into the pUC19 vector, and the DNA sequence was determined.
- The amplified sequence is 828 bp long, encoding 275 amino acid residues.
- Nucleotide and amino acid sequences showed 98.4% and 97.4% homology, respectively, to known sequences.
Impact:
- Provides a detailed genetic resource for maize RIPs.
- Facilitates further research into the function and evolution of RIPs in plants.
- Contributes to the development of enhanced crop protection strategies.