[Study on the effects of salicylic acid on taxol biosynthesis]

The influence of salicylic acid on the production of taxanes in plant cell culture was studied. Experimental results showed that addition of salicylic acid at concentration of 0.1 mg/L could enhance the production of taxol to three-fold. The concentration of 10-DAB and baccatin III was also increasing while taxol concentration increases under salicylic acid elicitation. On the basis of the kinetic analysis about the simplified taxol biosynthesis pathway, a probable reason that salicylic acid improves the rate of 10-DAB producing reaction was introduced. The results above can direct its inducing mechanic research and provide the basis of multiple-elicitors synergism. The concentration of taxol arrives at 39 mg.L-1 induced by salicylic acid with silver nitrate, being 150 percent of the sum of taxol obtained under elicitation of salicylic acid and silver nitrate respectively.