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Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Bacillus megaterium IWG3
K Yamamoto1, M Kusunoki, I Urabe
1Department of Chemistry, Nara Medical University, Kashihara, Nara 634-8521, Japan. kama@naramed-u.ac.jp
Acta Crystallographica. Section D, Biological Crystallography
|October 29, 2000
Abstract:
Glucose dehydrogenase from Bacillus megaterium IWG3 has been crystallized in the presence of NAD(+) using the hanging-drop vapour-diffusion method with PEG 2000 as the precipitant. Crystals belong to space group C2 and have unit-cell parameters a = 120.8 (1), b = 66.7 (1), c = 119.6 (1) A, beta = 93.25 (3) degrees with standard deviations in parentheses. Assumption of four subunits in the asymmetric unit gave the most probable Matthews coefficient V(M) of 2.1 A(3) Da(-1) (solvent content 41.7% by volume). X-ray diffraction data were collected to 1.7 A on a synchrotron-radiation source.