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Expression, crystallization and preliminary X-ray diffraction studies of recombinant Bacillus anthracis lethal factor
L Bernardi1, G Vitale, C Montecucco
1Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy.
Acta Crystallographica. Section D, Biological Crystallography
|October 29, 2000
Abstract:
The lethal factor (LF) produced by Bacillus anthracis is a Zn(2+)-dependent endopeptidase which specifically cleaves the N-terminal tail of several MAP kinase kinases (MAPKKs). The recombinant expression, purification and crystallization of LF and of an inactive mutant consisting of a single amino-acid substitution in the conserved catalytic site are reported here. Both proteins crystallize in the cubic space group I432.