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Related Experiment Videos

The gas 5 gene shows four alternative splicing patterns without coding for a protein.

G Raho1, V Barone, D Rossi

  • 1DIBIT, Istituto Scientifico San Raffaele, Milan, Italy.

Gene
|October 31, 2000
PubMed
Summary

The Gas5 gene, initially studied for cell cycle regulation, likely does not produce a protein. Novel mouse Gas5 messenger RNAs (mRNAs) lack open reading frames, suggesting a non-coding function.

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Area of Science:

  • Molecular Biology
  • Gene Expression Regulation
  • RNA Biology

Background:

  • The murine Gas5 gene was identified for its role in cell cycle growth arrest.
  • Initial studies identified two alternatively spliced transcripts.
  • Recent findings suggest Gas5 genes contain sequences homologous to small nucleolar RNAs involved in ribosomal RNA processing.

Purpose of the Study:

  • To identify and analyze the expression patterns of novel alternatively spliced mouse Gas5 messenger RNAs (mRNAs).
  • To investigate the protein-coding potential of the Gas5 gene.

Main Methods:

  • Identification and analysis of novel mouse Gas5 mRNA sequences.
  • Deduction of putative amino acid sequences from Gas5 complementary DNAs (cDNAs).
  • Antibody generation and Western blot analysis.

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  • Cloning and sequencing of the rat Gas5 gene.

    • Main Results:

    • Two novel alternatively spliced mouse Gas5 mRNAs were identified, lacking obvious open reading frames (ORFs).
    • No Gas5 protein was detected in cultured cells or murine tissue extracts using antibodies against deduced amino acid sequences.
    • Sequencing of the rat Gas5 gene revealed a stop codon within the putative ORF, truncating it after 13 amino acids.

    Conclusions:

    • The Gas5 gene likely does not encode a functional protein.
    • The identified novel Gas5 mRNAs may represent non-coding RNAs.
    • Further research is needed to elucidate the functional role of Gas5 non-coding transcripts.