Related Experiment Video
Updated: Oct 10, 2026

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
Published on: July 29, 2021
Vitamin C in the prevention of nitrate tolerance
1College of Pharmacy, University of New Mexico, Albuquerque 87131, USA.
Objective:
To evaluate the efficacy of vitamin C in preventing the development of nitrate tolerance.
Data Sources:
A MEDLINE search (from 1966 to July 2000) was conducted to identify relevant articles, with additional references obtained from the bibliographies of these articles.
Data Synthesis:
One possible mechanism of nitrate tolerance involves superoxide-induced deactivation of nitric oxide, providing the rationale for the use of antioxidants. Most published research concerning deactivation of nitric oxide has involved vitamin C; a summary of this information is presented here.
Conclusions:
Preliminary studies seem to support the role of vitamin C in attenuating the development of nitrate tolerance. Considering these findings, larger, long-term trials are necessary to further establish the role of vitamin C in this situation.
Related Concept Videos
Antianginal Drugs: Nitrates and β-Blockers
Organic nitrates, such as nitroglycerin, play a pivotal role. Once metabolized, they liberate nitric oxide, a molecular marvel. Nitric oxide triggers guanylyl cyclase and augments cGMP production. This biochemical cascade orchestrates the relaxation of vascular smooth muscles, ushering in vasodilation and enhancing coronary blood flow. Administered...
2° Amines to N-Nitrosamines: Reaction with NaNO2
Nitric Oxide Signaling Pathway
Vitamins
Antihypertensive Drugs: Vasodilators
Prevention of Further Absorption of Poison