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Updated: Jul 11, 2026

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Development of a time-resolved fluorometric detection system using diffusion-enhanced energy transfer

Koresawa¹, Kikuchi, Mizukami

¹Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan.

Analytical Chemistry

|October 31, 2000

Summary

A new detection system uses energy transfer and time-resolved fluorometry (TRF) for sensitive assays. This method detects changes in acceptor fluorescence, enabling high-resolution analysis of bioactive molecules.

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Last Updated: Jul 11, 2026

Fluorescence detection methods for microfluidic droplet platforms

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Published on: December 10, 2011

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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

Area of Science:

Biochemistry
Analytical Chemistry
Biophysics

Background:

Time-resolved fluorometry (TRF) is a sensitive detection technique.
Energy transfer mechanisms are crucial for developing novel biosensors.
Existing detection methods may lack sensitivity or require direct molecular attachment.

Purpose of the Study:

To develop a novel detection system combining energy transfer and TRF.
To utilize a europium chelate donor and a novel fluorophore acceptor (SNR1).
To enable detection of bioactive molecules through changes in acceptor fluorescence.

Main Methods:

Utilized a europium chelate as an energy donor and SNR1 as an energy acceptor.
Employed a diffusion-enhanced energy-transfer mechanism in solution.
Detected acceptor emission as long-lifetime fluorescence using TRF.
Monitored changes in acceptor fluorescence properties upon interaction with analytes.

Main Results:

Demonstrated energy transfer between donor and acceptor molecules without direct conjugation.
Observed long-lived sensitized emission from the acceptor, indicative of energy transfer.
Showcased the potential for detecting interactions via altered fluorescence properties.
Established a system applicable to various bioactive molecules through suitable acceptor selection.

Conclusions:

The developed system offers a simple, convenient, and high-resolution assay method.
The diffusion-enhanced energy transfer mechanism is effective for TRF-based detection.
This approach holds promise for sensitive detection and analysis of diverse bioactive molecules.