[Research on constructing phylogenetics trees of ruminants basing on the database of milk protein gene sequences]

Primers designed according to the sequences of four milk protein genes of cow Bos taurus (alpha-lactoalbumin, beta-lactoglobin, beta- and kappa-casein) were used to amplify the full length gene of alpha-lactalbumin in yak Bos grunniens (2999 bp), water buffalo Bubalus arnee bubalis (278 bp), partial sequence of this gene in red deer cervus elaphs xanthopygus (1582 bp), 5' and 3' flanking region of beta-lactoglobin gene (2167 bp and 1096 bp in length respectively), 5'-flanking region and exon VIII to exon IX of beta-casein gene (987 bp and 1096 bp in length respectively), exonIV of kappa-casein gene (780 bp). All the amplified DNA fragments were cloned and the Nt sequences were determined. Phylogenetic tree containing 20 species (or subspecies) of ruminantia suborder was constructed according to the partial sequence of kappa-casein gene exon IV (363 bp in length), which shows good monophyly of the Bovidae. And trees constructed according to other milk protein genes indicate that all the milk protein genes have good features for drawing phylogenetics tree at least among species belonging to different subfamilies.