Related Experiment Video
Updated: Aug 18, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Published on: June 17, 2019
Existence of two geographically-linked clonal lineages in the bacterial fish pathogen Photobacterium damselae subsp.
B Magariños1, A E Toranzo, J L Barja
1Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago, Santiago de Compostela, Spain.
Abstract:
In this work, we applied the random amplified polymorphic DNA (RAPD) technique to evaluate the genetic diversity in Photobacterium damselae subsp. piscicida (formerly Pasteurella piscicida), an important pathogen for different marine fish. Regardless of the oligonucleotide primer employed, the 29 isolates of Ph. damselae subsp. piscicida tested were separated into two groups, the RAPD-PCR analysis differentiated the European strains from the Japanese strains. The similarity between both groups estimated on the basis of the Dice coefficient was 75-80%. These results show that European and Japanese isolates of Ph. damselae subsp. piscicida, regardless of their host fish species, belong to two different clonal lineages. Our findings also indicate that RAPD profiling constitutes a useful tool for epidemiological studies of this fish pathogen.
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
Modern Molecular Taxonomy
Diversity of Protists I
Diversity of Protists II
Phylogenetic Species Concept in Microbiology

