Related Experiment Videos
Transendothelial migration of 27E10+ human monocytes
1Institute of Experimental Dermatology, University of Münster, von-Esmarch-Strasse 56, 48149 Münster, Germany.
International Immunology
|November 4, 2000
Summary
Myeloid-related proteins MRP8/14 (S100A8/S100A9) enhance monocyte migration during inflammation. These proteins promote transendothelial migration by increasing CD11b expression and facilitating movement through inflamed tissues.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Myeloid-related proteins MRP8 (S100A8) and MRP14 (S100A9) form a heterodimer involved in inflammatory processes.
- MRP8/14 is found in granulocytes and a monocyte subpopulation, and has been observed near transmigrating leukocytes in inflamed tissues.
Purpose of the Study:
- To investigate the role of MRP8/14 in monocyte transmigration across endothelium.
- To identify the specific monocyte subpopulation involved in fast migration and its underlying mechanisms.
Main Methods:
- Utilized the mAb 27E10 to identify and isolate a fast-migrating monocyte subpopulation (27E10+).
- Assessed MRP8/14 secretion, CD11b expression, and the effect of MRP8/14 heterodimers on monocytes.
- Employed antibody blocking to determine the role of MRP8 and MRP14 in monocyte transmigration.
Main Results:
- 27E10+ monocytes represent a fast-migrating subpopulation using ICAM-1-dependent mechanisms.
- MRP8/14 secretion was higher from 27E10+ monocytes after endothelial interaction.
- MRP14, but not MRP8, actively participated in monocyte transmigration, and MRP14/MRP8/14 increased CD11b expression.
Conclusions:
- MRP8/14 plays a significant role in monocyte recruitment to inflammatory sites.
- Released MRP8/14 enhances CD11b expression and facilitates transendothelial migration.
- MRP8/14 contributes to the inflammatory response by promoting monocyte infiltration.