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Inoculation of BALB/c mice with Lacazia loboi
S Madeira1, D V Opromolla, A d Belone
1Equipe Técnica de Microbiologia, Instituto Lauro de Souza Lima, Bauru, SP, Brasil.
Revista Do Instituto De Medicina Tropical De Sao Paulo
|November 4, 2000
Summary
BALB/c mice inoculated with Lacazia loboi developed lesions and fungal presence similar to human Jorge Lobo's Disease. This suggests BALB/c mice are a suitable model for studying the disease and testing treatments.
Area of Science:
- Mycology
- Infectious Diseases
- Immunology
Background:
- Previous studies established persistent granulomatous inflammation and viable Lacazia loboi (L. loboi) in Swiss mice.
- Jorge Lobo's Disease (JLD) is a fungal infection requiring suitable animal models for research.
Purpose of the Study:
- To evaluate BALB/c mice as a potential experimental model for studying Jorge Lobo's Disease.
- To assess the histopathological progression and fungal viability in BALB/c mice post-inoculation with L. loboi.
Main Methods:
- BALB/c mice were inoculated with a standardized suspension of L. loboi from a JLD patient biopsy.
- Animals were sacrificed at 1, 4, 7, and 10 months post-inoculation for analysis.
- Histopathological examination and fungal viability assessments were performed.
Main Results:
- Profuse infiltrates with histiocytes, giant cells, and numerous viable fungi were observed by 7 months.
- Macroscopic lesions indicative of JLD emerged around the 8th month post-inoculation.
- Fungal counts and viability remained significant throughout the observation period.
Conclusions:
- BALB/c mice exhibit histopathological and clinical features mirroring human Jorge Lobo's Disease.
- The BALB/c mouse model is proposed as valuable for investigating JLD pathogenesis and therapeutic interventions.