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Hepatotropism of nonenveloped DNA virus in rhesus monkey infected by transfusion-transmitted virus
Objective:
To study wether the nonenveloped DNA virus transmitted via blood transfusion is hepatotropic.
Methods:
Total DNA was extracted from tissues of 5 experimentally infected Rhesus monkeys. A dot hybridization was done with virus double DNA strand probe or single antisense strand probe.
Results:
Both single- and double-strand probes were hybridized with DNA of the liver, spleen, stomach, small intestine and colon. The virus was conformed present in most of all the organs when double-strand probe was used. The positive was noted only in the liver and small intestine when single-strand antisense probe was used, which showed that in liver and small intestine might have replicative intermediates of the virus.
Conclusion:
It suggests that nonenveloped DNA virus replicate in the liver and small intestine, so it might be hepatotropic.