Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intramolecular interactions regulate SAP97 binding to GKAP.

H Wu1, C Reissner, S Kuhlendahl

  • 1University of Alabama at Birmingham, Department of Neurobiology, 1719 Sixth Avenue South CIRC 589, Birmingham, AL 35294-0021, USA.

The EMBO Journal
|November 4, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Implementation of a fully digital histology course in the anatomical teaching curriculum during COVID-19 pandemic.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft·2021
Same author

Epistatic interaction of genetic depression risk variants in the human subgenual cingulate cortex during memory encoding.

Translational psychiatry·2014
Same author

The catheterism of the right chambers of the heart in humans.

Paris medical·2014
Same author

Common exonic missense variants in the C2 domain of the human KIBRA protein modify lipid binding and cognitive performance.

Translational psychiatry·2013
Same author

Short-term treatment with the GABAA receptor antagonist pentylenetetrazole produces a sustained pro-cognitive benefit in a mouse model of Down's syndrome.

British journal of pharmacology·2013
Same author

Treatment of chronic leukemia with urethra.

La Presse medicale·2010
Same journal

Chromosome condensation mechanically primes the nucleus for mitosis.

The EMBO journal·2026
Same journal

NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegans.

The EMBO journal·2026
Same journal

Assembly of the catalytic module and the rotor of human ATP synthase.

The EMBO journal·2026
Same journal

Substrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC.

The EMBO journal·2026
Same journal

Conformational changes of the baseplate regulating tail contraction of Staphylococcus phage 812.

The EMBO journal·2026
Same journal

Cellular assembly and functional resilience of the mammalian RNA exosome.

The EMBO journal·2026
See all related articles

Membrane-associated guanylate kinase homologs (MAGUKs) like SAP97 organize cellular junctions. Intramolecular interactions within SAP97 regulate its binding to GKAP, influencing cellular organization.

Area of Science:

  • Molecular and Cellular Biology
  • Structural Biology
  • Biochemistry

Background:

  • Membrane-associated guanylate kinase homologs (MAGUKs) are crucial for organizing cellular junctions.
  • SAP97 is a MAGUK protein that interacts with GUK-associated protein (GKAP).

Purpose of the Study:

  • To investigate the molecular mechanisms governing the interaction between SAP97 and GKAP.
  • To elucidate how intramolecular interactions within SAP97 regulate its binding to GKAP.

Main Methods:

  • Biochemical assays to study protein-protein interactions.
  • Generation of molecular models of SAP97 using crystal structure data.
  • Analysis of intramolecular interactions within SAP97.

Main Results:

Related Experiment Videos

  • The association of SAP97's SH3 domain and inter-domain sequences with its GUK domain inhibits GKAP binding.
  • N-terminal sequences of SAP97 facilitate GKAP binding through SH3 domain interaction.
  • Molecular modeling revealed a compact U-shaped conformation of SAP97, supporting intramolecular interactions.

Conclusions:

  • Intramolecular interactions within SAP97 play a key role in regulating its association with GKAP.
  • SAP97's conformation is dynamic and influenced by internal domain associations.
  • Understanding these mechanisms provides insights into the regulation of cellular junctions.