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Published on: July 9, 2012
Detection of extended-spectrum beta-lactamases in klebsiellae with the Oxoid combination disk method
M W Carter1, K J Oakton, M Warner
1Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom.
Abstract:
The Oxoid combination disk method for detecting extended-spectrum beta-lactamases (ESBLs) depends on comparing the inhibition zones of cefpodoxime (10-microg) and cefpodoxime-plus-clavulanate (10- plus 1-microg) disks. The presence of clavulanate enlarged the zones for all of 180 ESBL-producing klebsiellae by >/=5 mm, whereas zones for cefpodoxime-susceptible isolates and cefpodoxime-resistant isolates with AmpC and K1 beta-lactamases were enlarged by =1 mm. Good discrimination was achieved with either the NCCLS or the British disk method.
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