Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Early myotome specification regulates PDGFA expression and axial skeleton development.

M D Tallquist1, K E Weismann, M Hellström

  • 1Program in Developmental Biology, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

Development (Cambridge, England)
|November 4, 2000
PubMed
Summary

Platelet-derived growth factor A (PDGFA) signaling is crucial for axial skeleton development. Myogenic factors like Myf5 regulate PDGFA, impacting vertebral and rib formation in developing embryos.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Concordance between clinician-reported toxicities and patient-reported symptom severity in early-stage breast cancer: an analysis of the randomized phase III PANTHER trial.

ESMO open·2026
Same author

Prognostic role of serum thymidine kinase 1 kinetics during neoadjuvant chemotherapy for early breast cancer.

ESMO open·2021
Same author

Zoo animals as sentinels for Schmallenberg virus monitoring in Spain.

Veterinary microbiology·2020
Same author

Towards uterus tissue engineering: a comparative study of sheep uterus decellularisation.

Molecular human reproduction·2020
Same author

Dose tailoring of adjuvant chemotherapy for breast cancer based on hematologic toxicities: further results from the prospective PANTHER study with focus on obese patients.

Annals of oncology : official journal of the European Society for Medical Oncology·2018
Same author

First clinical experience with levodopa/carbidopa microtablets in Parkinson's disease.

Acta neurologica Scandinavica·2017

Area of Science:

  • Developmental Biology
  • Skeletal Biology
  • Molecular Genetics

Background:

  • Reciprocal signaling defects between myotome and sclerotome compartments in somites affect vertebral and rib formation.
  • Previous observations in PDGFRalpha and Myf5 mutant embryos indicated alterations in axial skeleton development.

Purpose of the Study:

  • To investigate the role of Platelet-Derived Growth Factor (PDGF) signaling in the developing somite.
  • To elucidate the relationship between myogenic determination factors and axial skeleton development.

Main Methods:

  • Analysis of PDGFA ligand expression in Myf5 null mutant embryos.
  • In vitro assessment of PDGFA promoter activity regulation by Myf5.
  • Somite micromass cultures and in vivo knock-in experiments to evaluate PDGFA's effect on chondrogenesis and skeletal development.

Related Experiment Videos

  • Comparison of PDGFA expression restoration using MyoD and myogenin knock-ins at the Myf5 locus.

    • Main Results:

    • PDGFA ligand expression was absent in the myotome of Myf5 mutant embryos, with Myf5 regulating PDGFA promoter activity.
    • Exogenous PDGFA enhanced chondrogenesis and increased vertebral and rib development in both in vitro and in vivo models.
    • MyoD knock-in fully restored PDGFA expression in the myotome, while myogenin had a lesser effect.

    Conclusions:

    • Growth factor signaling, specifically PDGFA, is vital for developing somites and axial skeleton formation.
    • Myogenic determination factors, particularly Myf5 and MyoD, play a significant role in orchestrating normal skeletal development through regulation of PDGFA.