Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Olfactory receptor trafficking involves conserved regulatory steps.

A A Gimelbrant1, S L Haley, T S McClintock

  • 1Department of Physiology, University of Kentucky Medical Center, Lexington 40536, USA.

The Journal of Biological Chemistry
|November 4, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk alleles of genes with monoallelic expression are enriched in gain-of-function variants and depleted in loss-of-function variants for neurodevelopmental disorders.

Molecular psychiatry·2017
Same author

Distribution of G-protein alpha subunits and neurotransmitter activation of g(alphai) and g(alphaq) in the brain of the lobster Homarus americanus.

The Journal of comparative neurology·2000
Same author

Lobster GABA receptor subunit expressed in neural tissues.

Journal of neuroscience research·2000
Same author

Lobster G-protein coupled receptor kinase that associates with membranes and G(beta) in response to odorants and neurotransmitters.

The Journal of comparative neurology·1999
Same author

A lobster phospholipase C-beta that associates with G-proteins in response to odorants.

The Journal of neuroscience : the official journal of the Society for Neuroscience·1999
Same author

Truncation releases olfactory receptors from the endoplasmic reticulum of heterologous cells.

Journal of neurochemistry·1999
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
Same journal

PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

The Journal of biological chemistry·2026
Same journal

Saturated cardiolipins are potent disruptors of inner mitochondrial membrane structure and function.

The Journal of biological chemistry·2026
Same journal

Phosphate release from myosin Va occurs after the initial powerstroke but before the secondary powerstroke associated with ADP-release.

The Journal of biological chemistry·2026
Same journal

Epigenetic silencing of miR-141 via core promoter methylation is associated with short-term bladder cancer progression.

The Journal of biological chemistry·2026
See all related articles

Olfactory receptors are challenging to express functionally. Differentiated odora cells, unlike undifferentiated ones, traffic these receptors to the cell surface, a process aided by the ODR-4 protein.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Olfactory receptors (ORs) are notoriously difficult to express functionally in heterologous systems.
  • They are often retained in the endoplasmic reticulum (ER) and require specific cellular environments for plasma membrane delivery.
  • This trafficking is crucial for understanding olfactory signaling and drug development.

Purpose of the Study:

  • To investigate the trafficking mechanisms of olfactory receptors in a novel cell line, odora.
  • To compare the trafficking behavior of ORs in undifferentiated versus differentiated odora cells.
  • To identify factors that facilitate OR plasma membrane delivery.

Main Methods:

  • Utilized the odora cell line, a novel olfactory cell line.

Related Experiment Videos

  • Employed differentiation protocols to alter odora cell maturation state.
  • Investigated receptor localization using microscopy and biochemical assays.
  • Assessed the role of the ODR-4 protein in OR trafficking.

    • Main Results:

    • Undifferentiated odora cells retain ORs in the ER, Golgi, and endosomes, but not at the plasma membrane.
    • Differentiated odora cells successfully traffic ORs to the plasma membrane.
    • Vomeronasal receptors (VN1 type) are also trafficked to the plasma membrane in differentiated odora cells.
    • The protein ODR-4 facilitates the trafficking of rat OR U131 in both odora and Chinese hamster ovary (CHO) cells.

    Conclusions:

    • Olfactory receptor trafficking involves at least two steps regulated by cellular maturation.
    • Differentiated odora cells provide a valuable model for studying OR trafficking.
    • The ODR-4 protein plays a conserved role in facilitating chemosensory receptor plasma membrane delivery.