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The human T-cell leukemia virus type I (HTLV-I) X region encoded protein p13(II) interacts with cellular proteins
1Laboratory of Immunogenetics, Twinbrook II Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12441 Parklawn Drive, Rockville, Maryland, 20852, USA.
Abstract:
Interactions between the Human T-cell leukemia virus type I (HTLV-I) gene product p13(II) and cellular proteins were investigated using the yeast two-hybrid system. Variant forms of p13(II) were derived from two HTLV-I molecular clones, K30p and K34p, that differ in both virus production and in vivo and in vitro infectivity. Two nucleotide differences between the p13 from K30p (p13K30) and K34p (p13K34) result in a Trp-Arg substitution at amino acid 17 and the truncation of the 25 carboxyl-terminal residues of p13K34. A cDNA library from an HTLV-I-infected rabbit T-cell line was screened with p13K30 and p13K34 as bait. Products of two cDNA clones, C44 and C254, interacted with p13K34 but not with p13K30. Interactions were further confirmed using the GST-fusion protein coprecipitation assay. Sequence analysis of C44 and C254 cDNA clones revealed similarities to members of the nucleoside monophosphate kinase superfamily and actin-binding protein 280, respectively. Further analysis of the function of these two proteins and the consequence of their interaction with p13 may help elucidate a role for p13 in virus production, infectivity, or the pathogenesis of HTLV-I.
Insights
Investigating Human T-cell leukemia virus type I (HTLV-I) p13(II) protein interactions revealed specific cellular protein binding. These findings offer insights into HTLV-I replication and pathogenesis.
Area of Science:
- Virology
- Molecular Biology
- Cellular Biology
Background:
- Human T-cell leukemia virus type I (HTLV-I) is a retrovirus associated with various diseases.
- The viral protein p13(II) is implicated in HTLV-I replication and pathogenesis, but its cellular interactions are not fully understood.
Purpose of the Study:
- To investigate the interactions between HTLV-I p13(II) and cellular proteins using variant forms of p13(II).
- To identify cellular proteins that bind to p13(II) and explore their functional significance.
Main Methods:
- Yeast two-hybrid system was employed to screen for interacting proteins.
- Variant p13(II) proteins (p13K30 and p13K34) differing in infectivity were used as bait.
- GST-fusion protein coprecipitation assays confirmed interactions.
- Sequence analysis identified interacting cellular proteins.
Main Results:
- Two cellular proteins, C44 and C254, were identified that interact with p13K34 but not p13K30.
- C44 showed similarity to nucleoside monophosphate kinase superfamily members.
- C254 showed similarity to actin-binding protein 280.
Conclusions:
- HTLV-I p13(II) interacts with specific cellular proteins, including nucleoside monophosphate kinase and actin-binding protein 280.
- These interactions may play a role in HTLV-I virus production, infectivity, or pathogenesis.
- Further research into these protein-protein interactions is warranted to elucidate p13's function.