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Different patterns of inducible nitric oxide synthase gene expression in ovarian carcinoma cell lines
1Department of Anesthesia and Critical Care Medicine, University of Innsbruck, Austria.
Background:
Stimulation of inducible nitric oxide synthase (iNOS) and release of nitric oxide by tumor cells may play a critical role in cancer development, either by exhibiting tumoricidal effects or by promoting tumor progression.
Materials And Methods:
In the present study, we investigated four ovarian carcinoma cell lines with respect to their iNOS expressing characteristics.
Results:
Following incubation with interferon-gamma, tumor necrosis factor-alpha and interleukin-1 beta, a marked stimulation of iNOS gene expression was found in SKOV-6 and OVCAR-3 cells, while only minor iNOS synthesis was detectable in HOC-7. Time-dependent accumulation of nitrite/nitrate in the culture supernatant indicated that the three cell lines were stimulated to produce and release nitric oxide. In contrast, no NO generation was observed in the fourth cell line under investigation, 2774.
Conclusion:
Due to the growth regulatory functions attributed to NO, variations in NO production may be of importance e.g. following interferon-gamma therapy in patients with ovarian cancer.
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