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pH and carbonate levels in developing enamel.

T Takagi1, T Ogasawara, J Tagami

  • 1Department of Biochemistry, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.

Connective Tissue Research
|November 4, 2000
PubMed
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Developing enamel pH zones correlate with proteolytic activity and crystal growth. Changes in pH influence enamel maturation by affecting protein decomposition and carbonate levels.

Area of Science:

  • Biomineralization
  • Dental Enamel Chemistry

Background:

  • Developing enamel exhibits dynamic pH changes along the tooth axis.
  • These pH variations define distinct neutral (N1, N2) and acidic (A1, A2) zones.

Purpose of the Study:

  • To correlate pH variations with proteolytic activity and crystal size in developing bovine enamel.
  • Investigate the chemical changes during enamel maturation.

Main Methods:

  • Enamel maturation stages identified using pH staining.
  • Crystal chemistry analyzed via thermogravimetry (TGA), ICP emission spectrometry, X-ray diffractometry (XRD), and infrared spectroscopy (IR).
  • Proteolytic enzyme activity assessed at different pH levels.

Main Results:

  • Optimal protease activity observed at pH 6.0, aligning with the A1 zone.

Related Experiment Videos

  • Organic enamel components decompose around 580°C.
  • Higher carbonate levels found in secretory stages; Ca/P molar ratio below stoichiometric 1.67.

    • Conclusions:

    • Enamel apatite crystal growth and maturation are linked to reduced carbonate levels.
    • Alternative calcification and protein decomposition processes appear crucial for enamel development.