Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Does amelogenin nanosphere assembly proceed through intermediary-sized structures?

A G Fincham1, W Leung, J Tan

  • 1Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, USA.

Connective Tissue Research
|November 4, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Triple Function of Amelogenin Peptide-Chitosan Hydrogel for Dentin Repair.

Journal of dental research·2023
Same author

Biomineralization of Enamel and Dentin Mediated by Matrix Proteins.

Journal of dental research·2021
Same author

An Evolutionarily Conserved Helix Mediates Ameloblastin-Cell Interaction.

Journal of dental research·2020
Same author

The Presence of MMP-20 Reinforces Biomimetic Enamel Regrowth.

Journal of dental research·2017
Same author

Amelogenin-Ameloblastin Spatial Interaction around Maturing Enamel Rods.

Journal of dental research·2016
Same author

Extracts of irradiated mature human tooth crowns contain MMP-20 protein and activity.

Journal of dentistry·2014
Same journal

Tissue zone dependence and asymmetric distribution of membrane ruffles of <i>in-situ</i> bovine chondrocytes.

Connective tissue research·2026
Same journal

Targeting extracellular collagen assembly to enhance functional recovery in composite neuromuscular trauma.

Connective tissue research·2026
Same journal

Ozonated water attenuates nucleus pulposus cell apoptosis and matrix degradation by suppressing NF-κB signaling in a rat model of <i>Staphylococcus aureus</i>-induced discitis.

Connective tissue research·2026
Same journal

Investigating the genetic basis of fibrotic pathway dysregulation in Dupuytren's contracture.

Connective tissue research·2026
Same journal

Kaempferol binds with NSUN2 to mediate RNA 5-methylcytosine modification of BMP2 in osteogenic differentiation.

Connective tissue research·2026
Same journal

Overexpression of NSUN3 suppresses ferroptosis in osteoarthritis chondrocytes by reducing m5C modification and expression of PCBP1 mRNA.

Connective tissue research·2026
See all related articles

Researchers found that amelogenin nanospheres, crucial for enamel biomineralization, assemble via smaller intermediate structures. These findings shed light on the molecular mechanisms driving tooth enamel formation.

Area of Science:

  • Biochemistry
  • Biomineralization
  • Materials Science

Background:

  • Nanosphere structures are proposed to organize and control enamel biomineralization at the ultrastructural level.
  • Observed nanosphere hydrodynamic radii (18-20 nm) suggest a composition of approximately 100 amelogenin monomers.

Purpose of the Study:

  • To investigate the molecular mechanism of amelogenin nanosphere assembly.
  • To determine the size distribution model that best describes recombinant murine amelogenin M179.

Main Methods:

  • Dynamic light scattering experiments were performed on recombinant murine amelogenin M179.
  • A newer size distribution algorithm was employed to analyze the data.

Main Results:

  • The size distribution data for M179 amelogenin are better described by a bimodal distribution model.

Related Experiment Videos

  • Intermediate structures (4-5 nm hydrodynamic radius, 4-6 amelogenin monomers) are proposed.
  • Assembly of 20 nm nanospheres is suggested to occur via inter-molecular hydrophobic interactions.

    • Conclusions:

    • Amelogenin nanosphere assembly involves intermediate sub-unit structures.
    • Hydrophobic interactions are implicated in the formation of the larger nanospheres observed in enamel matrix.