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Related Experiment Videos

Alpha(v)beta3 and alpha(v)beta5 integrin expression in meningiomas.

L Bello1, J Zhang, D C Nikas

  • 1Division of Neurosurgery and Brain Tumor Laboratory, Brigham and Women's Hospital, Children's Hospital and Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA.

Neurosurgery
|November 4, 2000
PubMed
Summary

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Integrins and angiogenesis.

Current topics in developmental biology·2004

Integrins alpha(v)beta3 and alpha(v)beta5 are expressed in meningiomas, suggesting a role in tumor angiogenesis. Alpha(v)beta3, in particular, may be linked to aggressive tumor behavior and is associated with vascular growth factors.

Area of Science:

  • Oncology
  • Cell Biology
  • Vascular Biology

Background:

  • Integrins mediate critical cellular functions, including angiogenesis, particularly in tumors.
  • Alpha(v)beta3 and alpha(v)beta5 integrins are key players in tumor-associated angiogenesis.
  • No prior data existed on integrin expression in meningiomas.

Purpose of the Study:

  • To investigate the expression and potential role of alpha(v)beta3 and alpha(v)beta5 integrins in meningiomas.
  • To correlate integrin expression with angiogenic factors and microvessel density in meningiomas.

Main Methods:

  • Western blot and immunohistochemistry were used to analyze 34 meningiomas.
  • Monoclonal antibodies LM609 and PG32 detected functional integrin heterodimers.
  • Expression of integrins, angiogenic factors (FGF2, VEGF), and vitronectin was assessed.

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Main Results:

  • Alpha(v)beta3 and alpha(v)beta5 integrins were detected in meningioma cells and vasculature.
  • Integrin expression correlated with angiogenic factors and microvessel density.
  • Alpha(v)beta3 showed stronger expression in aggressive benign meningiomas and was linked to vitronectin.

Conclusions:

  • Meningiomas express alpha(v)beta3 and alpha(v)beta5 integrins in both tumor cells and vasculature.
  • These integrins, especially alpha(v)beta3, are implicated in meningioma angiogenesis.
  • Findings suggest potential therapeutic targets for meningioma treatment.